AbsoluteIDQ® p400 HR kit
Best resolution
The AbsoluteIDQ® p400 HR kit is the first ready-to-use, standardized solution for broad lipid and metabolic profiling on Q Exactive™ mass spectrometers. It provides quantification of more than 400 metabolites and lipids from 11 compound classes covering central metabolic pathways.
AbsoluteIDQ® p400 HR kit
Best resolution
The AbsoluteIDQ® p400 HR kit is the first ready-to-use, standardized solution for broad lipid and metabolic profiling on Q Exactive™ mass spectrometers. It provides quantification of more than 400 metabolites and lipids from 11 compound classes covering central metabolic pathways.
Ready-to-use
- Implemented within one day
- All in one box: no method or reagent development required
- For a broadly established standard instrument base
High throughput & minimal sample volume
- Up to 240 samples per week and instrument
- 10 µL needed for most biofluids
(validated for human EDTA plasma)
Simple, guided, automated workflow
- Workflow manager guided procedure for enhanced process safety
- Automated signal identification, quantification, and technical validation
Superior data quality
- Quantitative results
- Proven analytical performance: excellent accuracy and precision
- Reproducibility proven by international ring trial
Standards and consumables
- Patented 96-well filter plates with integrated internal standards
- Calibration standards and quality controls
- Test samples for system suitability test
Methods and protocols
- Quick-start guide and user manual
- Protocols for different matrices
- Instrument-specific methods
WebIDQ™ – Workflow manager
- Cloud-based software (on-premise option also available)
- Complete experimental process guidance
- Automated quantification, technical validation and report generation
- MetaboINDICATOR™ with biologically relevant metabolite sums and ratios
LC column and MS systems
- Compatible with Thermo Exactive, Exactive focus, Exactive Plus, Exactive HF
- Validated LC column
- Compatible with ultra high performance liquid chromatography (UHPLC)
Kit workflow
A sample volume as small as only 10 µl is required. A multitude of sample types may be used with the kit, including blood (plasma or serum) and feces.
The workflow manager software MetIDQ™ guides users through the whole automated procedure from sample registration to high-quality results.
Small molecules
- Amino acid (20)
- Biogenic amines (21)
- Hexose (1)
Polar lipids
- Phosphatidylcholines (172)
- Lysophosphatidylcholines (24)
- Sphingomyelins (31)
- Ceramides (9)
Neutral lipids
- Acylcarnitines (11)
- Cholesteryl esters (14)
- Diglycerides (18)
- Triglycerides (42)
MetaboINDICATOR™
Translate metabolomics into knowledge with one click
Calculating and understanding metabolite sums and ratios makes your biological data interpretation easier and more comprehensive. That is why we created MetaboINDICATOR™ as an add-on tool to WebIDQ. With one click you can find out how metabolite sums and ratios are associated with related biological functions so your data has meaningful impact.
MetaboINDICATOR™ is more than an additional dimension to your data analysis; it unravels the scientific context behind your data. All in one click.
Related assets
brochure
AbsoluteIDQ® p400 HR kit
pdf ~ 0.8 MB
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brochure
biocrates kit technology
pdf ~ 6 MB
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biocrates services
pdf ~ 1.5 MB
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brochure
WebIDQ software
pdf ~ 2 MB
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Talk to an expert
biocrates life sciences ag
Eduard-Bodem-Gasse 8
6020 Innsbruck | AUSTRIA
Phone +43 512 579823
Fax +43 512 579823-329
biocrates inc.
26895 Aliso Creek Rd Ste B-426
Aliso Viejo, CA 92656-5301
Phone +1 774 424 8150
Operating hours: Mo – Fr | 9 am – 7 pm (EST)
Application: Functional Microbiomics and Foodomics
Unraveling the Interaction of Lifestyle, Microbiota, Metabolism, and Health
Investigations of the gut-liver, gut-heart, and gut-brain axis have become of increased interest as researchers become to understand the role of the microbiota and its effect on many organ systems. As metabolic profiles can be obtained from biofluids, metabolomics can provide information about microbiota-related processes where metagenomics studies may not be possible. The MxP® Quant 500 includes microbiota-derived secondary bile acids, which have different signaling properties than primary bile acids which are synthetized by the liver.